The Sekelsky Lab

pP{70w}

pP{70w} is a small vector for P element-mediated germline transformation. We have not yet used this vector in transformation. However, two other labs have reported problems obtaining transformants. We haven't determined what the source of this difficulty is. We have used this hsp70::white cDNA in other constructs, and it works well in our hands.

pP{70w} Sequence

CATGATGAAATAACATAAGGTGGTCCCGTCGAAAGCCGAAGCTTACCGAAGTATACACTT AAATTCAGTGCACGTTTGCTTGTTGAGAGGAAAGGTTGTGTGCGGACGAATTTTTTTTTG AAAACATTAACCCTTACGTGGAATAAAAAAAAATGAAATATTGCAAATTTTGCTGCAAAG CTGTGACTGGAGTAAAATTAATTCACGTGCCGAAGTGTGCTATTAAGAGAAAATTGTGGG AGCAGAGCCTTGGGTGCAGCCTTGGTGAAAACTCCCAAATTTGTGATACCCACTTTAATG ATTCGCAGTGGAAGGCTGCACCTGCAAAAGGTCAGACATTTAAAAGGAGGCGACTCAACG CAGATGCCGTACCTAGTAAAGTGATAGAGCCTGAACCAGAAAAGATAAAAGAAGGCTATA CCAGTGGGAGTACACAAACAGAGTAAGTTTGAATAGTAAAAAAAATCATTTATGTAAACA ATAACGTGACTGTGCGTTAGGTCCTGTTCATTGTTTAATGAAAATAAGAGCTTGAGGGAA AAAATTCGTACTTTGGAGTACGAAATGCGTCGTTTagagcagcagccgctagtggatccc cccgttattctctattcgttttgtgactctccctctctgtactattgctctctcactctg tcgcacagtaaacggcactctattctcgttgcttcgagagagcgcgcctcgaatgttcgc gaaaagagcgccggagtataaatagaggcgcttcgtcgacggagcgtcaattcaattcaa acaagCAAAGTGAACACATCGCGAAGCGTAAGCTGAGCAAACAAACAAGCGCAGCTGAAC AAGCTAAACAATCTGCAATAAAGTGCAAGTTAAAGTGAATCAATTAAAAGTAACCAACAA CCAAGTAATTAAACTAAAAACTGCAACTACTGAAATCAACCAAGAAGTCATTATTGAAGA CAAGAAGAGAACTCTgaataggtcgatagcgtcaattcggcacgaggcgtttcgtgacgaag ctccaagcggtttacgccatcaattaaacacaaagtgctgtgccaaaactcctctcgctt cttatttttgtttgttttttgagtgattgggtgggtgattggttttgggtgggtaagcag gggaaagtgtgaaaaatcccggcaATGGGCCAAGAGGATCAGGAGCTATTAATTCGCGGA GGCAGCAAACACCCATCTGCCGAGCATCTGAACAATTTGATATTTGAGATCCCCTATCATT GCAGGGTGACAGCGGACGCTTCGCAGAGCTGCATTAACCAGGGCTTCGGGCAGGCCAAAA ACTACGGCACGCTCCTGCCACCCAGTCCGCCGGAGGACTCCGGTTCAGGGAGCGGCCAAC TAGCCGAGAACCTCACCTATGCCTGGCACAATATGGACATCTTTGGGGCGGTCAATCAGC CGGGCTCCGGATGGCGGCAGCTGGTCAACCGGACACGCGGACTATTCTGCAACGAGCGAC ACATACCGGCGCCCAGGAAACATTTGCTCAAGAACGTTTGCGGCGTGGCCTATCCGGGCG AACTTTTGGCCGTGATGGGCAGTTCCGGTGCCGGAAAGACGACCCTGCTGAATGCCCTTG CCTTTCGATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGCTCAATGGCC AACCTGTGGACGCCAAGGAGATGCAGGCCAGGTGCGCCTATGTCCAGCAGGATGACCTCT TTATCGGCTCCCTAACGGCCAGGGAACACCTGATTTTCCAAGCCATGGTGCGGATGCCAC GACATCTGACCTATCGGCAGCGAGTGGCCCGCGTGGATCAGGTGATCCAGGAGCTTTCGC TCAGCAAATGTCAGCACACGATCATCGGTGTGCCCGGCAGGGTGAAAGGTCTGTCCGGCG GAGAAAGGAAGCGTCTGGCATTCGCCTCCGAGGCACTAACCGATCCGCCGCTTCTGATCT GCGATGAGCCCACCTCCGGACTGGACTCATTTACCGCCCACAGCGTCGTCCAGGTGCTGA AGAAGCTGTCGCAGAAGGGCAAGACCGTCATCCTGACCATTCATCAGCCGTCTTCCGAGC TGTTTGAGCTCTTTGACAAGATCCTTCTGATGGCCGAGGGCAGGGTAGCTTTCTTGGGCA CTCCCAGCGAAGCCGTCGACTTCTTTTCCTACGTGGGTGCCCAGTGTCCTACCAACTACA ATCCGGCGGACTTTTACGTACAGGTGTTGGCCGTTGTGCCCGGACGGGAGATCGAGTCCC GTGATCGGATCGCCAAGATATGGCGACAATTTTGCCATTAGCAAAGTAGCCCGGGATATG GAGCAGTTGTTGGCCACCAAAAATCTGGAGAAGCCACTGGAGCAGCCGGAGAATGGGTAC ACCTACAAGGCCACCTGGTTCATGCAGTTCCGGGCGGTCCTGTGGCGATCCTGGCTGTCG GTGCTCAAGGAACCACTCCTCGTAAAAGTGCGACTTATTCAGACAACGAAGATAAATAAA TTTCATTTAACTCGCGAATATTAATGAGATGCGAGTAACATTTTAATTTGCAGATGGTTG CCATCTTGATTGGCCTCATCTTTTTGGGCCAACAACTCACGCAAGTGGGTGTGATGAATA TCAACGGAGCCATCTTCCTCTTCCTGACCAACATGACCTTTCAAAACGTCTTTGCCACGA TAAATGTGTTCACCTCAGAGCTGCCAGTTTTTATGAGGGAGGCCCGAAGTCGACTTTATC GCTGTGACACATACTTTCTGGGCAAAACGATTGCCGAATTGCCGCTTTTTCTCACAGTGC CACTGGTCTTCACGGCGATTGCCTATCCGATGATCGGACTGCGGGCCGGAGTGCTGCACT TCTTCAACTGCCTGGCGCTGGTCACTCTGGTGGCCAATGTGTCAACGTCCTTCGGATATC TAATATCCTGCGCCAGCTCCTCGACCTCGATGGCGCTGTCTGTGGGTCCGCCGGTTATCA TACCATTCCTGCTCTTTGGCGGCTTCTTCTTGAACTCGGGCTCGGTGCCAGTATACCTCA AATGGTTGTCGTACCTCTCATGGTTCCGTTACGCCAACGAGGGTCTGCTGATTAACCAAT GGGCGGACGTGGAGCCGGGCGAAATTAGCTGCACATCGTCGAACACCACGTGCCCCAGTT CGGGCAAGGTCATCCTGGAGACGCTTAACTTCTCCGCCGCCGATCTGCCGCTGGACTACG TGGGTCTGGCCATTCTCATCGTGAGCTTCCGGGTGCTCGCATATCTGGCTCTAAGACTTC GGGCCCGACGCAAGGAGTAGccgacatatatccgaaataactgcttgtttttttttttta ccattattaccatcgtgtttactgtttattgccccctcaaaaagctaatgtaattatatt tgtgccaataaaaaaaaaaaaaaaaaaaaaaaaaaactcgagctctgtacatgtccgcgg tcgcgacgtacgcgtatcgatggcgccagctgcaggcggccgccatatgcatgcgaattc aggcctggatccactagttaacaaccggtacctctagaactatagctagcTTTGCGTACT CGCAAATTATTAAAAATAAAACTTTAAAAATAATTTCGTCTAATTAATATTATGAGTTAA TTCAAACCCCACGGACATGCTAAGGGTTAATCAACAATCATATCGCTGTCTCACTCAGAC TCAATACGACACTCAGAATACTATTCCTTTCACTCGCACTTATTGCAAGCATACGTTAAG TGGATGTCTCTTGCCGACGGGACCACCTTATGTTATTTCATCATGgtctggccattctca tcgtgagcttccgggtgctcgcatatctggctctaagacttcgcaggtggcacttttcgg ggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccg ctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagt attcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgttttt gctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtg ggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaa cgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtatt gacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgag tactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagt gctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggagga ccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgt tgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgta gcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccgg caacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcc cttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggt atcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacg gggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactg attaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaa cttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaa atcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaagga tcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccg ctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaact ggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccac cacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtg gctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcga acgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttccc gaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacg agggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctc tgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgcc ttcttcttgaactcgggctcggtgccagtatacctcaaatggttgtcgtacctctcatgg ttccgttacgccaacgagggtctgctgattaaccaatgggcggacgtggagccgggcgaa attagctgcacatcgtcgaacaccacgtgccccagttcgggcaaggtcatcctggagacg cttaacttctccgccgccgatctgccgctggactacgtgggtctggcc

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